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normal colonic mucosal epithelial cell line ncm460  (Genechem)

 
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    Genechem normal colonic mucosal epithelial cell line ncm460
    Normal Colonic Mucosal Epithelial Cell Line Ncm460, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+colonic+mucosal+epithelial+cell+line+ncm460/lentivirus/pm39198845-199-16-25
    Average 90 stars, based on 1 article reviews
    normal colonic mucosal epithelial cell line ncm460 - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: Gastrin-related circRNA_0017065 promotes the proliferation and metastasis of colorectal cancer through the miR-3174/RBFOX2 axis.
    Article Snippet: Four kinds of human CRC cells (HT29, HCT116, SW480 and LOVO), as well as a normal colonic mucosal epithelial cell line (NCM460), were purchased from Genechem (Shanghai, China).



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    Functional analysis of the representative gene APCDD1 in IRPS ( A ) Comparison of the APCDD1 expression level between CRC tissues and normal control tissues based on the GEPIA2 database; ( B ) The expression level of APCDD1 in CRC lines SW620, HT29 and SW480 was detected by qRT-PCR with the normal colon epithelial cell line <t>NCM460</t> as a control; ( C ) The siRNA interference efficiency of APCDD1 in HT29 cell line was detected by qRT-PCR; ( D ) CCK-8 assays were used to detect the effect of APCDD1 knockdown on HT29 cell proliferation; ( E and F ) Transwell assays were used to detect the effect of APCDD1 knockdown on HT29 cell migration ( E ) and invasion ( F ); ( G ) Apoptosis assays were used to detect the effect of APCDD1 knockdown on apoptosis of HT29 cells. * P < 0.05; ** P < 0.01; *** P < 0.001
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    Functional analysis of the representative gene APCDD1 in IRPS ( A ) Comparison of the APCDD1 expression level between CRC tissues and normal control tissues based on the GEPIA2 database; ( B ) The expression level of APCDD1 in CRC lines SW620, HT29 and SW480 was detected by qRT-PCR with the normal colon epithelial cell line <t>NCM460</t> as a control; ( C ) The siRNA interference efficiency of APCDD1 in HT29 cell line was detected by qRT-PCR; ( D ) CCK-8 assays were used to detect the effect of APCDD1 knockdown on HT29 cell proliferation; ( E and F ) Transwell assays were used to detect the effect of APCDD1 knockdown on HT29 cell migration ( E ) and invasion ( F ); ( G ) Apoptosis assays were used to detect the effect of APCDD1 knockdown on apoptosis of HT29 cells. * P < 0.05; ** P < 0.01; *** P < 0.001
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    Functional analysis of the representative gene APCDD1 in IRPS ( A ) Comparison of the APCDD1 expression level between CRC tissues and normal control tissues based on the GEPIA2 database; ( B ) The expression level of APCDD1 in CRC lines SW620, HT29 and SW480 was detected by qRT-PCR with the normal colon epithelial cell line <t>NCM460</t> as a control; ( C ) The siRNA interference efficiency of APCDD1 in HT29 cell line was detected by qRT-PCR; ( D ) CCK-8 assays were used to detect the effect of APCDD1 knockdown on HT29 cell proliferation; ( E and F ) Transwell assays were used to detect the effect of APCDD1 knockdown on HT29 cell migration ( E ) and invasion ( F ); ( G ) Apoptosis assays were used to detect the effect of APCDD1 knockdown on apoptosis of HT29 cells. * P < 0.05; ** P < 0.01; *** P < 0.001
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    Functional analysis of the representative gene APCDD1 in IRPS ( A ) Comparison of the APCDD1 expression level between CRC tissues and normal control tissues based on the GEPIA2 database; ( B ) The expression level of APCDD1 in CRC lines SW620, HT29 and SW480 was detected by qRT-PCR with the normal colon epithelial cell line <t>NCM460</t> as a control; ( C ) The siRNA interference efficiency of APCDD1 in HT29 cell line was detected by qRT-PCR; ( D ) CCK-8 assays were used to detect the effect of APCDD1 knockdown on HT29 cell proliferation; ( E and F ) Transwell assays were used to detect the effect of APCDD1 knockdown on HT29 cell migration ( E ) and invasion ( F ); ( G ) Apoptosis assays were used to detect the effect of APCDD1 knockdown on apoptosis of HT29 cells. * P < 0.05; ** P < 0.01; *** P < 0.001
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    Functional analysis of the representative gene APCDD1 in IRPS ( A ) Comparison of the APCDD1 expression level between CRC tissues and normal control tissues based on the GEPIA2 database; ( B ) The expression level of APCDD1 in CRC lines SW620, HT29 and SW480 was detected by qRT-PCR with the normal colon epithelial cell line <t>NCM460</t> as a control; ( C ) The siRNA interference efficiency of APCDD1 in HT29 cell line was detected by qRT-PCR; ( D ) CCK-8 assays were used to detect the effect of APCDD1 knockdown on HT29 cell proliferation; ( E and F ) Transwell assays were used to detect the effect of APCDD1 knockdown on HT29 cell migration ( E ) and invasion ( F ); ( G ) Apoptosis assays were used to detect the effect of APCDD1 knockdown on apoptosis of HT29 cells. * P < 0.05; ** P < 0.01; *** P < 0.001
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    Functional analysis of the representative gene APCDD1 in IRPS ( A ) Comparison of the APCDD1 expression level between CRC tissues and normal control tissues based on the GEPIA2 database; ( B ) The expression level of APCDD1 in CRC lines SW620, HT29 and SW480 was detected by qRT-PCR with the normal colon epithelial cell line NCM460 as a control; ( C ) The siRNA interference efficiency of APCDD1 in HT29 cell line was detected by qRT-PCR; ( D ) CCK-8 assays were used to detect the effect of APCDD1 knockdown on HT29 cell proliferation; ( E and F ) Transwell assays were used to detect the effect of APCDD1 knockdown on HT29 cell migration ( E ) and invasion ( F ); ( G ) Apoptosis assays were used to detect the effect of APCDD1 knockdown on apoptosis of HT29 cells. * P < 0.05; ** P < 0.01; *** P < 0.001

    Journal: BMC Cancer

    Article Title: A novel machine learning-based immune prognostic signature for improving clinical outcomes and guiding therapy in colorectal cancer: an integrated bioinformatics and experimental study

    doi: 10.1186/s12885-025-13437-0

    Figure Lengend Snippet: Functional analysis of the representative gene APCDD1 in IRPS ( A ) Comparison of the APCDD1 expression level between CRC tissues and normal control tissues based on the GEPIA2 database; ( B ) The expression level of APCDD1 in CRC lines SW620, HT29 and SW480 was detected by qRT-PCR with the normal colon epithelial cell line NCM460 as a control; ( C ) The siRNA interference efficiency of APCDD1 in HT29 cell line was detected by qRT-PCR; ( D ) CCK-8 assays were used to detect the effect of APCDD1 knockdown on HT29 cell proliferation; ( E and F ) Transwell assays were used to detect the effect of APCDD1 knockdown on HT29 cell migration ( E ) and invasion ( F ); ( G ) Apoptosis assays were used to detect the effect of APCDD1 knockdown on apoptosis of HT29 cells. * P < 0.05; ** P < 0.01; *** P < 0.001

    Article Snippet: The human CRC cell lines HT29, SW480 and SW620, as well as the human normal colon mucosal epithelial cell line NCM460, were purchased from KeyGEN BioTECH Corp., Ltd (China).

    Techniques: Functional Assay, Comparison, Expressing, Control, Quantitative RT-PCR, CCK-8 Assay, Knockdown, Migration